Thermo Fisher Scientific Phospho-ICAM-1 (Tyr512) Polyclonal Antibody
ICAM-1 Tyr512 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. Human, Mouse 및 Non-human primate 반응성. 고순도 액상 형태로 -20°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ICAM1 (Accession P05362), corresponding to amino acid residues around phosphorylated Tyr512 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818285 |
Product Specific Information
This antibody detects endogenous levels of ICAM1 only when phosphorylated at Tyrosine 512.
Target Information
ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region, and a cytoplasmic domain. ICAM-1 has seven potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102).
It binds to integrins of type CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and serves as a receptor for Rhinovirus. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B and T lymphocytes (low), monocytes, macrophages, dendritic cells, and neutrophils, with expression increasing upon inflammation. It is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in plasma and elevated in patients with various inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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