Thermo Fisher Scientific Phospho-ITCH / AIP4 (Tyr420) Polyclonal Antibody
Phospho-ITCH (Tyr420) 항체로 인간 및 생쥐 시료에서 인산화된 ITCH 단백질 검출에 적합. Western blot에 최적화된 다클론 항체. PBS 기반 완충액과 글리세롤 함유. -20°C 보관, 연구용 전용.
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Applications
- Western Blot (WB): 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ITCH (Accession Q96J02), corresponding to amino acid residues around phosphorylated Tyr420. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816551 |
Product Specific Information
This antibody detects endogenous levels of ITCH only when phosphorylated at Tyr420.
Target Information
Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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