Thermo Fisher Scientific Phospho-PCAF (Tyr729) Polyclonal Antibody
PCAF 단백질의 Tyr729 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot 검증 완료. Human, Mouse, Rat 반응성. PBS/glycerol buffer에 보관, -20°C 저장. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human KAT2B (Accession Q92831), corresponding to amino acid residues around phosphorylated Tyr729 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816556 |
Product Specific Information
This antibody detects endogenous levels of PCAF only when phosphorylated at Tyr729.
Target Information
PCAF (histone acetyltransferase KAT2B) functions as a histone acetyltransferase (HAT) to promote transcriptional activation. It has significant histone acetyltransferase activity with core histones (H3 and H4) and nucleosome core particles. PCAF also acetylates non-histone proteins such as ACLY, PLK4, and TBX5. It inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. PCAF also acts as a circadian transcriptional coactivator enhancing the activity of NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. It is involved in heart and limb development by mediating acetylation of TBX5 and regulating nucleocytoplasmic shuttling of TBX5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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