Takara Capturem trypsin digestion for mass spectrometry

Capturem Trypsin provides fast, efficient, and complete digestion of proteins at room temperature, in comparison to typical protein digestion workflows for mass spectrometry (MS) analyses that require four-hour or overnight digestion. By contrast, Capturem Trypsin`s rapid and simple protocol allows for an uninterrupted proteomic analysis workflow. This product, based on our novel Capturem technology, provides membrane-immobilized trypsin in a mini spin column or a 96-well plate format. Capturem Trypsin completely digests protein samples in just a few minutes, compared to the time-consuming incubations often required for in-solution digestion, and provides better efficiency with fewer autolyzed fragments*.

Capturem Trypsin provides fast, efficient, and complete digestion of proteins at room temperature, in comparison to typical protein digestion workflows for mass spectrometry (MS) analyses that require four-hour or overnight digestion. By contrast, Capturem Trypsin`s rapid and simple protocol allows for an uninterrupted proteomic analysis workflow. This product, based on our novel Capturem technology, provides membrane-immobilized trypsin in a mini spin column or a 96-well plate format. Capturem Trypsin completely digests protein samples in just a few minutes, compared to the time-consuming incubations often required for in-solution digestion, and provides better efficiency with fewer autolyzed fragments. In addition, these Capturem spin columns and plates make it easier to avoid the over-digestion commonly seen with in-solution digests. Process up to 80 µg of protein or 25 µg of antibody on each well or column.

More effective proteomic analysis

Protein digestion using Capturem Trypsin is more effective than in-solution digestion for preparing samples for MS analysis because it eliminates long digestion times, which may lead to oxidation and other protein modifications, as well as increased autolysis, which can make peptide and protein identification more difficult and less reproducible.

Fast, easy protocol

Capturem Trypsin includes mini spin columns or 96-well plates and an activation buffer. Capturem protein digestion is performed using a fast, easy protocol. Just load 200 µl of activation buffer, spin for 1–2 minutes, and then load your protein sample and spin for another minute to elute peptides that are ready for downstream analysis.

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Overview

• Digest protein samples in less than four minutes instead of overnight
• Generate fewer autolyzed trypsin fragments and protein modifications
• Get an uninterrupted proteomics analysis workflow
• Get more effective peptide and protein identification in MS analysis
• Process up to 80 µg of protein or 25 µg of antibody on each well or column

*All the mass spectrometry grade products have been manufactured with our new porcine dimethylated trypsin to increase stability and prevent autolysis more efficiently. Our older product, Capturem Trypsin Miniprep Kit, Cat. # 635722, manufactured with bovine trypsin will be discontinued soon.

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Applications

• Protein characterization
• Proteomic analysis
• Mass spectrometry sample preparation
• Antibody characterization

References

Robinson, M.R., et al., Improving the throughput of immunoaffinity purification and enzymatic digestion of therapeutic proteins using membrane-imobilized reagent technology. Analyst 145, 3,148-3,156 (2020).

Additional product information

Please see the product′s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.