Takara Rapid Protein G-based antibody purification

Capturem Protein G columns and plates combine the specific antibody binding properties of Protein G with Capturem technology to provide rapid, high-quality purification and screening of monoclonal and polyclonal antibodies. Where resin columns fall short, Capturem Protein G yields highly pure monoclonal and polyclonal antibodies in just 15 minutes, from loading the sample through elution.

Capturem Protein G columns and plates combine the specific antibody binding properties of Protein G with Capturem technology to provide rapid, high-quality purification and screening of monoclonal and polyclonal antibodies. Where resin columns fall short, Capturem Protein G yields highly pure monoclonal and polyclonal antibodies in just 5–15 minutes, from loading the sample through elution. Short residence times greatly reduce the possibility of antibody aggregation or loss of activity, giving downstream applications a higher probability of success.

Protein G is a cell wall protein from the bacterium Staphylococcus aureus with a high affinity for the Fc region of monoclonal and polyclonal IgG-type antibodies from several different species, e.g., mouse, rat, human, etc. This product uses a recombinant Protein G that was engineered to remove the albumin-binding domain, as well as the cell wall and cell membrane binding domains, in order to improve specificity towards the Fc region of antibodies. Capturem kits use a novel high-capacity membrane-based affinity technology that has previously been used to purify highly concentrated his-tagged proteins in just minutes at room temperature, via miniprep columns, maxiprep columns, 24-well plates, and 96-well plates.

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Overview

• Fast and convenient: room-temperature protocol, complete in under 15 minutes, without incubation
• High purity: small column bed volumes trap fewer contaminants and allow large-volume washes
• High quality: short residence times reduce the possibility of aggregation or loss of activity
• Flexible: purify a wide range of antibodies

Note: Capturem Protein G 24-Well Plate - HC is optimized to give you an increased yield compared to our previous version.

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Applications

• Antibody purification
• Immunoprecipitation

Approximate yields for Capturem Protein G formats

*Yields vary depending upon the sample type, species, and antibody isotype.

**This yield was obtained when spiking 1 mg of mouse IgG1 in HEK293 cell supernatant.

Isotype affinity of Protein A and Protein G

Key: +++ strong binding; ++ medium binding; + weak binding; − no binding

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.