Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), NovaFluor Red 710, eBioscience
마우스 CD19를 인식하는 eBio1D3 (1D3) 단클론 항체로, NovaFluor Red 710 형광체가 결합된 형광세포분석용 항체입니다. B세포 표면 CD19 검출에 사용되며, CellBlox Plus Blocking Buffer와 함께 제공되어 최적의 형광 신호를 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | ≤ 0.1 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio1D3 (1D3) |
| Conjugate | NovaFluor™ Red 710 |
| Excitation / Emission Max | 637 / 713 nm |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| RRID | AB_3098918 (AB_2896751) |
Additional Available Formats
- Unconjugated
- Alexa Fluor 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV/Violet series (395–805)
- eFluor 450, 506, 660
- FITC
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright series (436–780)
- NovaFluor Blue, Violet, Yellow series (510–810)
- Functional Grade
- Custom conjugation available
Product Specific Information
The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed on B cells throughout development except plasma cells, and on follicular dendritic cells. CD19 forms a complex with CD21, CD81, and MHC class II, modulating BCR signaling and B cell activation.
Each product includes:
- 1 vial of NovaFluor conjugate
- 1 vial of CellBlox Plus Blocking Buffer
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; ≤ 0.1 µg/test recommended.
Optimal titration is advised.
Important Notes:
- NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
- Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
- CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
- Add 5 µL of blocking buffer per sample before combining with cells.
NovaFluor Technology:
Based on Phiton™ technology with engineered fluorescent signatures designed to minimize spillover and spread.
- Excitation: 639 nm
- Emission: 710 nm
- Laser: 633–640 nm (Red)
Target Information
CD19 is a transmembrane glycoprotein of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and some myeloid leukemia cells. CD19 functions as a co-receptor with BCR, lowering the activation threshold for antigen stimulation and regulating B cell proliferation. Mutations cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.
For Research Use Only. Not for use in diagnostic procedures.
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