Thermo Fisher Scientific Human SCF, AOF Recombinant Protein
Human SCF 재조합 단백질로, E. coli 발현 시스템에서 생산된 고순도(>98%) 단백질입니다. 세포 증식 활성(ED50 < 2.0 ng/mL)을 가지며, Western blot, IHC, ELISA 등 다양한 분석에 적합합니다. Carrier-free 형태로 제공되며, 장기 보관 시 -20°C 보관을 권장합니다.
- 카탈로그번호
- PHC6034
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Human SCF recombinant protein contains 165 amino acids |
| Molecular Weight | 18.4 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >98% by SDS-PAGE and HPLC |
| Endotoxin Concentration | <1 EU/µg |
| Activity | ED50 < 2.0 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Carrier-Free
Reconstitution:
Briefly centrifuge the vial before opening. Reconstitute SCF in sterile, distilled water to a concentration of 0.1–1.0 mg/mL. Further dilutions should be made in aqueous buffers supplemented with carrier protein.Storage:
The lyophilized preparation is stable at 2–8°C but should be stored at -20°C for long-term storage, preferably desiccated.
After reconstitution, stable for up to one week at 2–8°C.
For maximal stability, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
Stem Cell Factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways.
Binding of SCF to the c-Kit receptor induces receptor homodimerization and activates signaling pathways such as PI-3, PLC-γ, Jak/STAT, and MAP kinase.
SCF expression promotes mast cell survival and the release of histamine, pro-inflammatory cytokines, and chemokines.
Inhibition of the SCF/c-Kit pathway reduces histamine levels, mast cell and eosinophil infiltration, IL-4 production, and airway hyperresponsiveness, suggesting potential therapeutic relevance in inflammatory diseases such as asthma.
At least two isoforms of SCF are known to exist.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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