Thermo Fisher Scientific CD15/FUT4 (Reed-Sternberg Cell Marker) Monoclonal Antibody (SPM119)
CD15/FUT4 (SPM119) 단클론 항체로 Reed-Sternberg 세포 마커 검출에 사용됩니다. IHC, ICC/IF, Flow Cytometry에 적용 가능하며, 인간 시료 반응성을 가집니다. 단백질 A/G 정제, 보존제 미함유 PBS 용액 형태로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM119 |
| Immunogen | U937 histiocytic cell line |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins, and proteoglycans of various cell types, including granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and various tumor cells.
Variants include sialyl-Lewis X or sulphated Lewis X. Cells with high Le(x) surface expression exhibit calcium-dependent Le(x)-Le(x) aggregation, involved in processes such as embryo compaction and teratocarcinoma cell autoaggregation. Sialyl-Le(x) and sialyl-Le(a) serve as selectin ligands. CD15 expression is widely used to confirm the diagnosis of Hodgkin’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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