Thermo Fisher Scientific CD15/FUT4 (Reed-Sternberg Cell Marker) Monoclonal Antibody (Leu-M1 (MMA))
CD15/FUT4 단일클론 항체로 Reed-Sternberg 세포의 표지에 사용됩니다. IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합합니다. 인간 시료 반응성, Mouse IgM 항체이며, 단백질 A/G로 정제된 액상 형태입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Leu-M1 (MMA) |
| Immunogen | U937 histiocytic cell line |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins, and proteoglycans of various cell types such as granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and various tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X.
Cells with high Le(x) expression exhibit calcium-dependent self-aggregation, involved in embryo compaction and teratocarcinoma cell autoaggregation.
Sialyl-Le(x) and sialyl-Le(a) are selectin ligands.
CD15 expression is widely used to confirm Hodgkin’s disease diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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