Thermo Fisher Scientific CD15/FUT4 (Reed-Sternberg Cell Marker) Monoclonal Antibody (Leu-M1 (MMA))
CD15/FUT4 단클론 항체로 Reed-Sternberg 세포 마커로 사용. 인간 시료에 반응하며 IHC, ICC, Flow Cytometry 등에 적합. Protein A/G 정제, 액상 형태, 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Leu-M1 (MMA) |
| Immunogen | U937 histiocytic cell line |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on various glycolipids, glycoproteins, and proteoglycans of multiple cell types including granulocytes, mast cells, monocytes, macrophages, gastric mucosa, nervous system cells, and tumor cells.
Variants such as sialyl-Lewis X or sulphated Lewis X exist. Cells with high Le(x) expression exhibit strong calcium-dependent self-aggregation, important in embryo compaction and teratocarcinoma cell autoaggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands. CD15 expression is widely used to confirm Hodgkin’s disease diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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