Thermo Fisher Scientific CPLX1/2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)KDMGKMLGGDEEKD, corresponding to amino acid residues 14-27 of rat Complexin-1, Intracellular, N-terminus if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CPLX1/2,uniProtId:P63041-1,ncbiNodeId:10114,antigenRange:14-27,antigenLength:134,antigenImageFileName:ANR-011-200UL_CPLX12_P63041-1_Rabbit.svg,antigenImageFileNamePDP:ANR-011-200UL_CPLX12_P63041-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

Complexins (1 through 4) are small cytosolic proteins that bind tightly to a complex of SNARE proteins to regulate synaptic vesicle membrane fusion via its interaction withsynaptotagmin. Complexins activate the quick release of neurotransmitters, triggered by calcium release. They also regulate the spontaneous release of neurotransmitters. Complexins are evolutionarily conserved in mammals, Drosophila, and Caenorhabditis elegans. They are divided into four structural domains: flexible N- and C-terminal domains, an accessory domain, and a central alpha-helical domain. The N-terminal domain plays a role in activation of fast synchronous release in murine neurons and in isolated chromaffin cells. The C-terminal domain binds to phospholipids and is important for regulating spontaneous release in neuronal cultures and suppressing Ca2+-independent fusion in vitro. The accessory domain is necessary for regulating spontaneous release and the central domain is required for all functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.