Thermo Fisher Scientific CD43 Monoclonal Antibody (eBioR2/60), PE, eBioscience

Thermo Fisher Scientific CD43 Monoclonal Antibody (eBioR2/60), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• View 26 publications

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• FITC
• Request custom conjugation

Product Specific Information

The eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in bone marrow, thymocytes, mature T cells, NK cells, myeloid lineage cells, and platelets. CD43 binds to CD54 and plays a role in adhesion and B cell survival.

While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects both isoforms. R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. S7 and 1B11 recognize distinct epitopes differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤ 0.5 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test (empirically determined).
Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on most hematopoietic cells. It forms a repulsive barrier that interferes with cellular adhesion but can also promote leukocyte aggregation. Through interaction with actin-binding proteins ezrin and moesin, CD43 regulates T-cell morphology and cell-cell interactions during lymphocyte traffic.

CD43 signaling enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 induces tumor suppressor p53, but in p53 and ARF deficiency, it promotes tumor proliferation. CD43 is an important modulator of leukocyte functions. Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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