Thermo Fisher Scientific Phospho-EphB2 (Tyr298) Polyclonal Antibody
Human 및 Rat에서 반응하는 Rabbit Polyclonal 항체로, Tyr298에서 인산화된 EphB2 단백질을 특이적으로 인식합니다. Western blot에 적합하며, lambda-phosphatase 처리로 면역표지 차단이 확인됩니다. Affinity chromatography로 정제된 액상 형태로, -20°C에서 보관합니다.
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Applications and Tested Dilution
- Western Blot (WB): 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr298 of chicken EPHB2 conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage buffer | 0.01M HEPES (pH 7.5), 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage conditions | -20 °C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2095689 |
Product Specific Information
This antibody is predicted to react with bovine, canine, human, mouse, non-human primate, Xenopus, and zebrafish based on 100% sequence homology.
It specifically recognizes the ~46 kDa EphrinB protein phosphorylated at Tyr298. Immunolabeling can be blocked by lambda-phosphatase treatment.
Target Information
EPHB2 is a receptor tyrosine kinase belonging to the ephrin receptor family. Members of the Eph family play critical roles in biological processes such as nervous system development, angiogenesis, and neural synapse formation and maturation.
Ephrins are divided into two classes: ephrin-A (EFNA), which are GPI-anchored, and ephrin-B (EFNB), which are transmembrane proteins. Eph receptors are categorized into two groups based on extracellular domain sequence similarity and ligand-binding affinity. EphB4 binds to ephrin-B2 and is essential for vascular development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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