Thermo Fisher Scientific STIM1 Polyclonal Antibody
STIM1 단백질을 인식하는 Rabbit Polyclonal Antibody로 다양한 종(Human, Mouse 등)에 반응. Western blot과 ICC/IF에 적합하며, 항원 친화 크로마토그래피로 정제됨. 액상 형태로 1 mg/mL 농도, 장기보관 시 -20°C 권장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide made to a C-terminal region with residues 600–685 of the human protein (Uniprot#Q13586) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 30% glycerol |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2197881 |
Product Specific Information
The target sequence has 93% sequence homology with porcine and 79% sequence homology with xenopus.
Suggested positive control: antigen standard for STIM1 (transient overexpression lysate), HeLa whole cell lysate.
Target Information
STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane.
Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm.
STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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