Thermo Fisher Scientific STIM1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:200

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide made to a C-terminal region with residues 600-685 of the human protein.[Uniprot#Q13586] if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:STIM1,uniProtId:Q13586-1,ncbiNodeId:9606,antigenRange:600-685,antigenLength:685,antigenImageFileName:PA1-46217_STIM1_Q13586-1_Rabbit.svg,antigenImageFileNamePDP:PA1-46217_STIM1_Q13586-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 30% glycerol

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2197881

Product Specific Information

The target sequence has 93% sequence homology with porcine and 79% sequence homology with xenopus.

Suggested positive control: antigen standard for STIM1 (transient overexpression lysate), HeLa whole cell lysate.

Target Information

STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.