Thermo Fisher Scientific Klotho Polyclonal Antibody, PeproTech
Human Klotho 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western Blot 및 ELISA에 최적화됨. 항원 친화 크로마토그래피로 정제되었으며, 고순도 재조합 단백질 기반 면역원 사용. -20°C 보관, 연구용 전용.
- 카탈로그번호
- 500-P296-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Klotho Polyclonal Antibody, PeproTech
Thermo Fisher Scientific Klotho Polyclonal Antibody, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human Klotho |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2930278 |
Product Specific Information
AA Sequence of recombinant protein:
EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYH
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Klotho. Anti-Human Klotho-specific antibody was purified by affinity chromatography employing an immobilized Human Klotho matrix.
Sandwich ELISA:
To detect Human Klotho by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. In conjunction with PeproTech Biotinylated Anti-Human Klotho (500-P296Bt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Human Klotho.
Western Blot:
To detect hKlotho by Western Blot, use at 0.1–0.2 µg/mL. In conjunction with compatible secondary reagents, the detection limit for Recombinant hKlotho is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
KLOTHO is a systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein abundant in kidney and brain. In mice, KLOTHO deficiency leads to phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy, along with growth retardation, short life-span, and infertility. Overexpression of KLOTHO extends life span by inhibiting insulin/IGF1 signaling. It regulates calcium/phosphorus homeostasis by inhibiting active vitamin D synthesis and is identified as a potential tumor suppressor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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