Thermo Fisher Scientific Phospho-Histone H3 (Ser10) Monoclonal Antibody (K.872.3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1:100

-

Immunocytochemistry (ICC/IF)

1:400

View 2 publications 2 publications

Flow Cytometry (Flow)

1:25

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

K.872.3

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pSer10 of human histone H3 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Histone H3,uniProtId:P68431-1,ncbiNodeId:9606,antigenRange:10,antigenLength:136,antigenImageFileName:MA5-15220_Histone_H3_P68431-1_House_mouse.svg,antigenImageFileNamePDP:MA5-15220_Histone_H3_P68431-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:Histone H3,uniProtId:Q71DI3-1,ncbiNodeId:9606,antigenRange:10,antigenLength:136,antigenImageFileName:MA5-15220_Histone_H3_Q71DI3-1_House_mouse.svg,antigenImageFileNamePDP:MA5-15220_Histone_H3_Q71DI3-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

50 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_11008586

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other phosphorylated histones or acetylated histone H3.

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.