Thermo Fisher Scientific Phospho-Histone H3 (Ser10) Monoclonal Antibody (K.872.3)
Phospho-Histone H3 (Ser10)에 특이적인 마우스 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 사용 가능. 고순도의 Affinity chromatography 정제 제품으로 -20°C 보관. 다른 히스톤 변형과 교차 반응 없음.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:400 | View 2 publications |
| Flow Cytometry (Flow) | 1:25 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | K.872.3 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pSer10 of human histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 50 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_11008586 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other phosphorylated histones or acetylated histone H3.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones can alter chromatin structure or facilitate the binding of transcription factors. The N-terminal tail of histone H3 can undergo various modifications such as methylation, acetylation, and phosphorylation, influencing cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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