Thermo Fisher Scientific CNTF Polyclonal Antibody, PeproTech
Rat CNTF 단백질을 인식하는 Rabbit Polyclonal 항체로, Western Blot과 ELISA에 최적화되어 있습니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 용액에 동결건조 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 500-P79-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CNTF Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Rat CNTF |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930767 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat CNTF. Anti-Rat CNTF-specific antibody was purified by affinity chromatography employing an immobilized Rat CNTF matrix.
Sandwich ELISA:
To detect Rat CNTF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat CNTF (500-P79Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Rat CNTF.
Western Blot:
To detect Rat CNTF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat CNTF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system, where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It is a potent survival factor for neurons and oligodendrocytes and may help reduce tissue destruction during inflammatory attacks.
A mutation in this gene causes ciliary neurotrophic factor deficiency, though not directly linked to neurological disease. A read-through transcript variant composed of ZFP91 and CNTF sequences has been identified but is believed to be non-coding. Similar read-through transcription has been observed in mice.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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