Thermo Fisher Scientific CNTF Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat CNTF. Anti-Rat CNTF-specific antibody was purified by affinity chromatography employing an immobilized Rat CNTF matrix.

Sandwich ELISA:
To detect Rat CNTF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat CNTF (500-P79Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Rat CNTF.

Western Blot:
To detect Rat CNTF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat CNTF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system, where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It is a potent survival factor for neurons and oligodendrocytes and may help reduce tissue destruction during inflammatory attacks.
A mutation in this gene causes ciliary neurotrophic factor deficiency, though not directly linked to neurological disease. A read-through transcript variant composed of ZFP91 and CNTF sequences has been identified but is believed to be non-coding. Similar read-through transcription has been observed in mice.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.