Thermo Fisher Scientific CNTF Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat CNTF. Anti-Rat CNTF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Rat CNTF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat CNTF (500-P79) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Rat CNTF.

Western Blot:
To detect Rat CNTF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat CNTF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P79BT-1MG will be provided as 2 × 500 µg.

Target Information

The protein encoded by this gene is a polypeptide hormone whose actions are restricted to the nervous system, where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene causing aberrant splicing leads to ciliary neurotrophic factor deficiency, though not directly related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified but is thought to be non-coding. Similar read-through transcription has been observed in mouse.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.