Thermo Fisher Scientific CNTF Polyclonal Antibody, Biotin, PeproTech
Rat CNTF 단백질을 인식하는 Biotin 결합 토끼 폴리클로날 항체로, Western blot과 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 고순도 재조합 Rat CNTF로 면역화하여 제작되었습니다. 신경세포 연구용으로 적합합니다.
- 카탈로그번호
- 500-P79BT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CNTF Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Rat CNTF |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930771 |
Product Specific Information
AA Sequence of recombinant protein:
AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat CNTF. Anti-Rat CNTF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Rat CNTF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat CNTF (500-P79) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Rat CNTF.
Western Blot:
To detect Rat CNTF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat CNTF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Packaging:
500-P79BT-1MG will be provided as 2 × 500 µg.
Target Information
The protein encoded by this gene is a polypeptide hormone whose actions are restricted to the nervous system, where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene causing aberrant splicing leads to ciliary neurotrophic factor deficiency, though not directly related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified but is thought to be non-coding. Similar read-through transcription has been observed in mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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