Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136)
Mouse NK1.1 단백질을 인식하는 PK136 단클론 항체로, Flow Cytometry와 IHC에 적합. C57BL, FVB/N, NZB 마우스에서 NK 및 NKT 세포 검출에 사용. Protein G 정제, 1 mg/mL 농도의 액상 포맷. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 8 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/1 million cells | View 6 publications |
| ChIP assay (ChIP) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PK136 |
| Immunogen | NK-1+ cells from mouse spleen and bone marrow |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2296673 |
Additional Formats
Product Specific Information
Clone PK136 is specific for mouse NK1.1, also known as NKR-PIC and Ly-55.
Mouse NK1.1 is expressed on NK and NKT cells in strains C57BL, FVB/N, and NZB.
Reports indicate PK136 also binds to NKR-PIB on SJL/K NK cells.
In addition to Flow and IHC, this clone has been reported to work in in-vitro and in-vivo depletion assays.
Target Information
NK1.1 (Killer cell lectin-like receptor subfamily B, member 1, KLRB1, NKR-P1A, CD161)
NK1.1 refers to Natural Killer (NK) cells, lymphocytes that mediate cytotoxicity and secrete cytokines upon immune stimulation.
Genes of the C-type lectin superfamily, including the rodent NKRP1 family, are expressed by NK cells and may regulate NK cell function.
The KLRB1 protein contains:
- An extracellular domain with C-type lectin motifs
- A transmembrane domain
- A cytoplasmic domain
KLRB1 (NKR-P1A or CD161) is a type II membrane protein with an external C terminus and recognizes Lectin Like Transcript-1 (LLT1) as its functional ligand.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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