Thermo Fisher Scientific Bcl-2 Monoclonal Antibody (10C4), eBioscience
Mouse 및 Rat Bcl-2 단백질을 인식하는 단클론 항체로, apoptosis 조절 연구에 적합. Western blot, Flow cytometry, Immunoprecipitation에 사용 가능. 높은 순도(>90%)와 안정적 액상 제형을 제공하며, 다양한 형광체 결합 형식 지원.
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Bcl-2 Monoclonal Antibody (10C4), eBioscience
Thermo Fisher Scientific Bcl-2 Monoclonal Antibody (10C4), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–10 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | Assay-Dependent | View 4 publications |
| Immunoprecipitation (IP) | Assay-Dependent | – |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10C4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865255 |
Available Conjugated Formats
Product Specific Information
The 10C4 monoclonal antibody reacts with mouse and rat Bcl-2, also known as B cell lymphoma 2. Bcl-2 is the founding member of the Bcl-2 family of proteins that regulate cell death and survival. Members include pro-apoptotic proteins (BAD, Bax, Bim) and anti-apoptotic proteins (Bcl-2, Bcl-xL, Mcl-1).
Bcl-2 inhibits apoptosis by preventing cytochrome c release from mitochondria. Mutation or overexpression of Bcl-2 due to chromosomal translocation contributes to various cancers.
In thymocytes, Bcl-2 is highly expressed in CD4–CD8– double negative and CD4+ or CD8+ single positive cells, but diminished in CD4+CD8+ double positive thymocytes.
Applications Reported:
Used for intracellular staining followed by flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).
Fluorochrome-conjugated 10C4 is recommended for intracellular flow cytometry.
Applications Tested:
Validated for western blot at 1–10 µg/mL; titration recommended for optimal assay performance.
Additional Characteristics:
- Purity: >90% (SDS-PAGE)
- Aggregation: <10% (HPLC)
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
BCL-2 regulates apoptosis, functioning to inhibit or promote cell death. Family members dimerize to modulate apoptosis.
Bag1 forms a heterodimer with BCL-2, enhancing its anti-apoptotic effect. Bax and Bak promote cytochrome c release, initiating apoptosis.
Constitutive expression of BCL-2 (e.g., via translocation to Ig heavy chain locus) is linked to follicular lymphoma.
In follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, while normal centers are negative.
Two transcript variants exist, differing at the C-terminal end. Overexpression of BCL-2 is associated with cancers such as B-cell lymphoma and prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.