Thermo Fisher Scientific Bax Monoclonal Antibody (6A7), eBioscience

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Description:
The 6A7 antibody reacts with mouse, rat, monkey, and human Bax (20 kDa). It shows no cross-reaction with Bcl-2 or Bcl-xL proteins. Bcl-2, Bax, and Bcl-xL are members of a protein family that regulates apoptosis. While Bcl-2 and Bcl-xL inhibit apoptosis, overexpression of Bax promotes programmed cell death. A human Bax peptide sequence (aa 12–24) was used as the immunogen.

Applications Reported:
Reported for use in immunoblotting (WB).

Applications Tested:
Tested by Western blot and can be used at approximately 1 µg/mL. It is recommended to titrate for optimal performance in the assay of interest.

Purity:

90%, determined by SDS-PAGE.

Aggregation:
<10%, determined by HPLC.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

BAX is a member of the Bcl-2 family and plays an important role in apoptosis regulation. While Bcl-2 functions as an anti-apoptotic protein, BAX is pro-apoptotic. Regulation involves homo- and heterodimerization of BAX and Bcl-2 isoforms. The Bax gene encodes isoforms Bax alpha (21 kDa) and Bax beta (24 kDa), both containing BH1, BH2, and BH3 domains. Bax beta lacks a hydrophobic transmembrane domain. Bcl-2 isoforms differ in coding regions, with Bcl-2 beta (22 kDa) being shorter than Bcl-2 alpha (26 kDa). Overexpression of BAX promotes cell death via homodimer or heterodimer formation with other BCL-2-related proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.