Thermo Fisher Scientific Bax Monoclonal Antibody (6A7), eBioscience
Bax 단백질을 특이적으로 인식하는 6A7 클론 단일클론 항체. Western blot 등 다양한 응용에 사용 가능. 인간, 마우스, 랫드, 영장류 반응성. 세포사멸 연구용으로 적합. 4°C 보관, PBS 버퍼에 0.09% sodium azide 포함.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Bax Monoclonal Antibody (6A7), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 8 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | - | View 2 publications |
| Immunoprecipitation (IP) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6A7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468389 |
Product Specific Information
Description:
The 6A7 antibody reacts with mouse, rat, monkey, and human Bax (20 kDa). It shows no cross-reaction with Bcl-2 or Bcl-xL proteins. Bcl-2, Bax, and Bcl-xL are members of a protein family that regulates apoptosis. While Bcl-2 and Bcl-xL inhibit apoptosis, overexpression of Bax promotes programmed cell death. A human Bax peptide sequence (aa 12–24) was used as the immunogen.
Applications Reported:
Reported for use in immunoblotting (WB).
Applications Tested:
Tested by Western blot and can be used at approximately 1 µg/mL. It is recommended to titrate for optimal performance in the assay of interest.
Purity:
90%, determined by SDS-PAGE.
Aggregation:
<10%, determined by HPLC.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
BAX is a member of the Bcl-2 family and plays an important role in apoptosis regulation. While Bcl-2 functions as an anti-apoptotic protein, BAX is pro-apoptotic. Regulation involves homo- and heterodimerization of BAX and Bcl-2 isoforms. The Bax gene encodes isoforms Bax alpha (21 kDa) and Bax beta (24 kDa), both containing BH1, BH2, and BH3 domains. Bax beta lacks a hydrophobic transmembrane domain. Bcl-2 isoforms differ in coding regions, with Bcl-2 beta (22 kDa) being shorter than Bcl-2 alpha (26 kDa). Overexpression of BAX promotes cell death via homodimer or heterodimer formation with other BCL-2-related proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.