Thermo Fisher Scientific IkB zeta Monoclonal Antibody (LK2NAP), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The LK2NAP monoclonal antibody reacts with mouse IkB zeta (INAP, MAIL). IkBz is a member of the IkB family of cytoplasmic proteins and transcription factors, sharing structural homology with Bcl-3. It is induced in monocytes, macrophages, and B cells in response to TLR and IL-1R signaling.

In NK cells, IkBz is rapidly upregulated by IL-12 and IL-18 and is necessary for IFN-γ production. In T cells, IkBz is not required for IFN-γ or IL-4 production by Th1/Th2 cells. However, in Th17 cells, IkBz acts with RORα and RORγt to upregulate IL-17A, IL-17F, IL-21, IL-22, and IL-23R expression.

Applications Reported:
This LK2NAP antibody is reported for use in Western blotting.

Applications Tested:
Tested by Western blotting on reduced lysates at >1 µg/mL. It is recommended to titrate the antibody for optimal performance.

Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

I-kappa-B-zeta (also known as MAIL and INAP) is an ankyrin-repeat-containing nuclear protein homologous to the IkappaB family member Bcl-3. Transcription is upregulated by TLR ligands, IL-1, and IL-6 in B-lymphocytes and monocytes/macrophages, but only faintly in T-lymphocytes, fibroblasts, and endothelial cells.
IkappaB-zeta preferentially associates with the NF-kappaB subunit p50 and inhibits the DNA binding of the p65/p50 heterodimer and the p50/p50 homodimer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.