Thermo Fisher Scientific p19ARF Monoclonal Antibody (5-C3-1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

5 µ/gmL

-

Immunohistochemistry (Frozen) (IHC (F))

5 µg/mL

-

Immunocytochemistry (ICC/IF)

1:500-1:1,000

-

Flow Cytometry (Flow)

1 µg/mL

-

Immunoprecipitation (IP)

1:10-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

5-C3-1

Immunogen

Synthetic peptide containing amino acids 54-75 of the murine p19ARF. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:p19ARF,uniProtId:Q64364-1,ncbiNodeId:10090,antigenRange:54-75,antigenLength:169,antigenImageFileName:MA1-16665_p19ARF_Q64364-1_Rat.svg,antigenImageFileNamePDP:MA1-16665_p19ARF_Q64364-1_Rat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568665

Product Specific Information

This antibody does not react with human or Golden hamster.

Armenian hamster has not been tested.

Target Information

In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various onocoproteins via the p53 regulatory loop. These studies and others display the importance of p19ARF in cell cycle control and tumor suppression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.