Thermo Fisher Scientific c-Myc Monoclonal Antibody (9E11)
다양한 종에서 c-Myc 단백질을 검출하는 마우스 단클론 항체. Western blot, IHC, ICC, Flow cytometry 등 다중 응용 가능. 고순도 Protein A/G 정제 및 안정한 액상 형태. 암 연구 및 세포 증식 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | 11 publications |
| Immunohistochemistry (IHC) | – | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100 | – |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 1 publication |
| Flow Cytometry (Flow) | 1:200–1:400 | 1 publication |
| ELISA | 1:100–1:2,000 | – |
| Immunoprecipitation (IP) | 2 µg/mg lysate | 6 publications |
| ChIP assay (ChIP) | 2 µg/500 µg extract | 8 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Rat, Yeast |
| Published Species | Human, Mouse, Rat, Shrew, Yeast |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9E11 |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to residues A(408) E E Q K L I S E E D L(420) of human c-Myc |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_568496 |
Product Specific Information
- Suggested positive control: antigen standard for MYC (transient overexpression lysate), HL-60 cells or breast tumors, HL-60 whole cell lysate, human breast carcinoma whole cell lysate.
Target Information
The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays critical roles in:
- Cellular proliferation, differentiation, apoptosis, and cell cycle progression
- Phosphorylation at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK
- Vasculogenesis and angiogenesis in tumor development
Antibodies against c-Myc epitopes recognize overexpressed proteins containing Myc epitope tags fused to either N- or C-termini of target proteins.
Amplification or translocation of the c-Myc gene is associated with multiple cancers, including lung, breast, colon carcinomas, and Burkitt’s lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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