Thermo Fisher Scientific IL-1 beta Monoclonal Antibody (CRM56), eBioscience

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

View 5 publications 5 publications

ELISA (ELISA)

1-4 µg/mL

View 6 publications 6 publications

Neutralization (Neu)

Assay-Dependent

View 3 publications 3 publications

Functional Assay (Functional)

Assay-Dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

CRM56

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 647
• Brilliant UV 395
• Brilliant Violet 650
• FITC
• Functional Grade
• PE
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468400

Product Specific Information

Description: The CRM56 antibody reacts with human and baboon interleukin-1 beta; (IL-1 beta). IL-1 beta is a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. IL-1 has effects on B cells, promoting cell proliferation and immunoglobulin synthesis.

Applications Reported: The CRM56 antibody has been reported for use in intracellular staining for flow cytometric analysis, cytokine neutralization, and ELISA. Fluorochrome conjugated CRM56 is recommended for use in intracellular staining for flow cytometry. Functional Grade purified CRM56 (Product # 16-7018) is recommended for use in functional assays.

Applications Tested: The CRM56 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interleukin-1 beta (IL-1 beta) in combination with the biotin CRM57 (Product # 13-7016-81) antibody for detection and recombinant human IL-1 beta (14-8018) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

The Functional Grade Purified CRM56 antibody has been tested for neutralization of IL-1 beta bioactivity.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.