Thermo Fisher Scientific IL-1 beta Monoclonal Antibody (CRM56), eBioscience

Thermo Fisher Scientific IL-1 beta Monoclonal Antibody (CRM56), eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats Available:

• Alexa Fluor 647
• Brilliant UV 395
• Brilliant Violet 650
• FITC
• Functional Grade
• PE
• Custom conjugation available

Product Specific Information

The CRM56 antibody reacts with human and baboon interleukin-1 beta (IL-1β), a 17 kDa factor secreted primarily by monocytes. IL-1β influences T-helper cells (inducing IL-2 secretion and receptor expression) and B cells (promoting proliferation and immunoglobulin synthesis).

Applications Reported:
CRM56 has been reported for intracellular staining (flow cytometry), cytokine neutralization, and ELISA. Fluorochrome-conjugated CRM56 is recommended for flow cytometry; Functional Grade purified CRM56 is recommended for functional assays.

Applications Tested:
CRM56 has been tested as a capture antibody in sandwich ELISA for human IL-1β, using biotin CRM57 as detection antibody and recombinant human IL-1β as standard. Recommended ELISA capture concentration: 1–4 µg/mL. Standard curve range: 1000 pg/mL–8 pg/mL.

Functional Grade Purified CRM56 has been validated for IL-1β bioactivity neutralization.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Interleukin-1 beta (IL-1β) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. Synthesized as a 31 kDa inactive pro-form, it is cleaved by caspase-1 into the active 17 kDa form following inflammasome activation. IL-1β is secreted via a non-classical pathway and signals through IL-1RI and IL-1RII receptors. Its activity is regulated by IL-1 Receptor Antagonist (IL-1RA). IL-1β plays a key role in innate immune responses and inflammation, and elevated levels are linked to chronic inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.