Thermo Fisher Scientific TROP2 Monoclonal Antibody (M005)
TROP2 단백질을 인식하는 Mouse Monoclonal Antibody (Clone M005). Western blot, ICC/IF, ELISA, IP 등에 사용 가능. Human 시료 반응성. Protein G 정제, 액상 형태, -20°C 보관. 연구용 전용 시약.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M005 |
| Immunogen | Clone M005 was generated from a proprietary antigen related to the extracellular region of human Trop-2 from the A431 epidermoid carcinoma cell line. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942663 |
Product Specific Information
Clone M005 detects a 50–65 kDa band corresponding to the molecular mass of Trop-2 on SDS-PAGE immunoblots of native human A431, MDA-MB-231, and MCF-7 cell lysates.
The antibody also detects denatured Trop-2 with lower affinity, so lower dilutions should be used for denatured western blot.
Clone M005 is suitable for Western blot, immunocytochemistry, ELISA capture, and immunoprecipitation, as well as for detecting live unfixed cells.
Target Information
TROP2 is a carcinoma-associated antigen defined by the monoclonal antibody GA733.
This antigen belongs to a family of type I membrane proteins and functions as a cell surface receptor that transduces intracellular calcium signals.
Mutations in the TROP2 gene cause gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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