Thermo Fisher Scientific Human EGF, AOF Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• Immunohistochemistry (IHC): Assay-dependent
• ELISA: Assay-dependent
• Functional Assay: Assay-dependent
• Control (Ctrl): Assay-dependent

Product Specifications

Product Specific Information

Carrier-Free
Reconstitution: Briefly centrifuge vial before opening to collect contents at the bottom. Reconstitute EGF in sterile, distilled water to a concentration of 0.1–1.0 mg/mL. Further dilutions should be made in aqueous buffers supplemented with carrier protein.

Storage:
Lyophilized preparation is stable at 2–8°C, but should be stored at -20°C for long-term storage, preferably desiccated.
After reconstitution, stable for up to one week at 2–8°C. For maximal stability, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles.

Target Information

EGF (Epidermal Growth Factor) binds to the EGF receptor (EGFR), a 170 kDa protein kinase, initiating multiple cellular pathways. EGFR forms homo- or heterodimers with other family members, each activating distinct signaling cascades via SH2-containing effector proteins.

EGF is widely expressed in kidney, cerebrum, prostate, and salivary glands. It acts as a potent mitogenic factor, recruiting adapter proteins like GRB2 to activate downstream signaling cascades such as RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT.

Research suggests EGF may also activate the NF-κB signaling pathway. Defects in the EGF gene are linked to hypomagnesemia type 4, and dysregulation has been associated with certain cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.