Thermo Fisher Scientific Human FGF-basic (FGF-2/bFGF), AOF Recombinant Protein

Applications and Tested Dilution

Product Specifications

Product Specific Information

Carrier-Free

Reconstitution:
Briefly centrifuge the vial before opening. Reconstitute FGF Basic in sterile distilled water to 0.1–1.0 mg/mL. Further dilutions should be made in aqueous buffers with carrier protein.

Storage:

• Lyophilized preparation is stable at 2–8°C; for long-term storage, keep at -20°C (preferably desiccated).
• After reconstitution, stable for up to one week at 2–8°C.
• For maximal stability, aliquot and store at -20°C.
• Avoid repeated freeze-thaw cycles.

Target Information

FGF2 (FGFb, fibroblast growth factor basic) is a member of the fibroblast growth factor family that binds high-affinity transmembrane receptors to regulate cell proliferation and angiogenesis.
Five isoforms exist, differing in localization and function:

• The 18 kDa isoform is cytosolic and receptor-dependent.
• The 22, 22.5, 24, and 34 kDa isoforms are nuclear and may signal independently of membrane receptors.

FGF2 plays key roles in limb and nervous system development, wound healing, and tumor growth.
The human FGF2 gene is located on chromosome 4q.
Translation from multiple initiation codons produces isoforms with distinct properties:

• CUG-initiated isoforms act intracrinally (nuclear localization).
• AUG-initiated isoform acts paracrinally/autocrinally (cytosolic localization).

Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.