Thermo Fisher Scientific VEGF Receptor 3 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is placenta or breast carcinoma.

Target Information

Vascular endothelial growth factor (VEGF) is a key regulator of blood vessel development in embryos and angiogenesis in adult tissues.
Unlike VEGF, the related VEGFC stimulates the growth of lymphatic vessels through its specific lymphatic endothelial receptor VEGFR-3.
In 1998, Dumont showed that targeted inactivation of the VEGFR-3 gene in mice resulted in defective blood vessel development in early embryos.
Vasculogenesis and angiogenesis occurred, but large vessels became abnormally organized with defective lumens, leading to fluid accumulation and cardiovascular failure at embryonic day 9.5.
VEGFR3 plays an essential role in the development of the embryonic cardiovascular system before the emergence of lymphatic vessels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.