Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), Brilliant Violet 711, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.25 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 786
• eFluor 450
• eFluor 506
• eFluor 570
• eFluor 615
• eFluor 660
• FITC
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 (345 and 395 kDa) are expressed in dividing cells and detectable during G1, S, G2, and mitosis, but absent in resting cells (G0). During interphase, Ki-67 localizes to the nucleus and redistributes to chromosomes during mitosis, associating with heterochromatin-bound proteins such as CBX3. Ki-67 expression is used as a marker for proliferating cell fractions in tumor studies.
This antibody also recognizes canine Ki-67.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-diluted and tested using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00). Recommended use: 5 µL (0.25 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.

Fluorochrome Information:
Brilliant Violet™ 711 (BV711) emits at 713 nm and is for use with violet (405 nm) laser-equipped cytometers.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to minimize non-specific interactions.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
Compatibility: Not compatible with methanol-based fixation.
Excitation/Emission: 407 nm / 713 nm (Violet Laser).

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and heterochromatin regions and exhibits phase-dependent localization: perinucleolar in G1, nuclear matrix in G2, and chromosomal in M phase.
Ki-67 serves as a proliferation marker, absent in G0 cells, and correlates with tumor grade and proliferation rate. Commonly used to evaluate neoplastic tissue proliferation and neuronal cell cycling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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