Thermo Fisher Scientific Nestin Monoclonal Antibody (10C2), Alexa Fluor 488, eBioscience
인간 Nestin 단백질을 인식하는 10C2 클론의 Alexa Fluor 488 결합 단일클론 항체. 줄기세포, 신경계 및 종양 조직에서 Nestin 발현 연구에 적합. 면역형광, 면역조직화학, 유세포분석 등에 활용 가능. 높은 특이성과 형광 안정성을 제공.
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Thermo Fisher Scientific Nestin Monoclonal Antibody (10C2), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (IHC) | Assay-Dependent | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | View 3 publications |
| Flow Cytometry (Flow) | Assay-Dependent | View 3 publications |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10C2 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1834347 |
Product Specific Information
The monoclonal antibody 10C2 recognizes human nestin (residues 1464–1614).
Nestin is a 220–240 kDa Class VI intermediate filament protein expressed in stem cells of the developing nervous system. Expression is also found in pancreatic islet and mesenchymal precursors, as well as in cells with angiogenic potential. Upon differentiation, nestin filaments are replaced by cell type-specific intermediate filament proteins such as GFAP. Expression has been identified in various cancer cells including glioblastomas and pancreatic tumors. No cross-reactivity to rodent nestins has been observed with 10C2 antibody.
Applications Reported:
This antibody is suitable for microscopy, immunocytochemistry, immunohistochemical staining, and flow cytometric analysis.
Applications Tested:
Validated for immunocytochemistry of formaldehyde-fixed and permeabilized cells at ≤10 µg/mL. Optimal dilution should be determined experimentally.
Fluorescence Properties:
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing CNS and early embryonic neuroepithelial stem cells. It serves as a key marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Nestin is also a superior angiogenic marker used to evaluate tumor neovascularity. Specific antibodies to human Nestin facilitate characterization of progenitor cells, enhance therapeutic potential of neural stem/progenitor cells, and aid in identifying neuroepithelial tumor cells. Nestin expression has been noted in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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