Thermo Fisher Scientific CDK1 Monoclonal Antibody (POH-1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Human, Non-human primate

Published species

Bovine, Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

POH-1

Immunogen

Bacterially expressed full-length human Cdk1 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CDK1,uniProtId:P06493-1,ncbiNodeId:9606,antigenRange:1-297,antigenLength:297,antigenImageFileName:MA1-19057_CDK1_P06493-1_House_mouse.svg,antigenImageFileNamePDP:MA1-19057_CDK1_P06493-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1075472

Product Specific Information

This antibody detects Cdk1 (p34Cdc2; an intracellular antigen). Staining of Cdk1 reflects the proliferating potential of respective tissue.

This antibody will not cross-react with Drosophila, mouse, Xenopus or rat.

Target Information

CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.