Thermo Fisher Scientific CD44/HCAM Std (Cancer Stem Cell Marker) Monoclonal Antibody (HCAM/918)
CD44/HCAM 단일클론 항체(HCAM/918)는 암 줄기세포 마커로서 인간 및 비인간 영장류 반응성을 가지며, 다양한 면역분석(WB, IHC, ICC, Flow 등)에 적합합니다. 단백질 A/G로 정제된 액상 형태이며, PBS(pH 7.4)에 보존제로 없이 제공됩니다.
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Thermo Fisher Scientific CD44/HCAM Std (Cancer Stem Cell Marker) Monoclonal Antibody (HCAM/918)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Functional Assay | Assay-dependent |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HCAM/918 |
| Immunogen | Stimulated human leukocytes (Target: CD44, UniProt ID: P16070-1, Antigen Range: 1–742) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD44 cell surface antigen is a 100 kDa type I transmembrane glycoprotein widely expressed on human leukocytes, white matter of the brain, and some epithelial cells of the intestine and breast.
Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues.
CD44 acts as a receptor for hyaluronic acid (HA) and plays roles in cell-cell interactions, adhesion, and migration. It is involved in lymphocyte activation, recirculation, and homing.
Expression of CD44 may be upregulated in certain carcinomas, potentially related to metastatic potential. It is expressed by hematopoietic and non-hematopoietic cells, epithelial tissues, and filopodia in cultured keratinocytes.
Bone marrow myeloid cells and memory T cells show high CD44 expression, while peripheral B and T cells can upregulate CD44 in response to stimulation.
CD44 transcripts undergo complex alternative splicing, resulting in diverse isoforms with distinct functions. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus, and CD44 may be implicated in tumor metastasis formation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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