Thermo Fisher Scientific CD44/HCAM Std (Cancer Stem Cell Marker) Monoclonal Antibody (HCAM/918)
CD44/HCAM 단클론 항체(HCAM/918)는 암 줄기세포 마커로 사용되며 다양한 면역실험(WB, IHC, ICC, Flow 등)에 적합합니다. 인간 및 비인간 영장류 반응성을 가지며, 단백질 A/G로 정제된 액상 형태입니다. 4°C 보관, 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Functional Assay | Assay-dependent |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HCAM/918 |
| Immunogen | Stimulated human leukocytes |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD44 cell surface antigen is a 100 kDa type I transmembrane glycoprotein widely expressed on human leukocytes, white matter of the brain, and certain epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues.
CD44 acts as a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, adhesion, and migration. It plays roles in lymphocyte activation, recirculation, and homing. Expression of CD44 may be upregulated in certain carcinomas and is associated with metastatic potential.
CD44 is expressed by hematopoietic and non-hematopoietic cells, epithelial tissues, and filopodia in cultured keratinocytes. Bone marrow myeloid cells and memory T cells express CD44 at high levels, while peripheral B and T cells can upregulate CD44 upon stimulation.
Transcripts of the CD44 gene undergo complex alternative splicing, generating many distinct isoforms. This splicing contributes to the structural and functional diversity of CD44 protein.
Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 may also be linked to tumor metastasis formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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