Thermo Fisher Scientific PLSCR1 Monoclonal Antibody (13A6 [TEC-23])

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Flow Cytometry (Flow)

Assay-Dependent

-

Immunoprecipitation (IP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

13A6 [TEC-23]

Immunogen

Pooled lipid rafts isolated from RBL-2H3 cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PLSCR1,uniProtId:P58195-1,ncbiNodeId:10114,antigenRange:1-335,antigenLength:335,antigenImageFileName:MA5-28630_PLSCR1_P58195-1_House_mouse.svg,antigenImageFileNamePDP:MA5-28630_PLSCR1_P58195-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_2745589

Product Specific Information

This antibody binds to an intracellular epitope of rat Phospholipid Scramblase 1 (PLSCR1), an 37-49 kDa protein, accelerating bidirectional movement of plasma membrane phospholipids under conditions of elevated calcium.

Western Blot: Both reducing and non-reducing conditions.

Target Information

The calcium-dependent plasma membrane protein phospholipid scramblase 1 (PLSCR1) contributes to the transbilayer movement of phosphatidylserine and other membrane phospholipids upon influx of calcium into the cytosol. This movement results in plasma membrane phospholipid remodelling and surface exposure of phosphatidylserine in injured or apoptotic cells, which leads to cell death. Interferons and other cytokines induce expression of PLSCR1, implying that PLSCR1 also functions in cytokine signaling pathways. EGF stilmulation results in tyrosine phosphorylation of PLSCR1 on tyrosines 69 and 74, which allows it to interact with Shc, and thereby connecting Src kinase activation to stimulation of the EGF receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.