Thermo Fisher Scientific SIRT5 Monoclonal Antibody (OTI2A11), TrueMAB
SIRT5 단백질을 표적으로 하는 마우스 단클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 사용 가능. 고순도 친화 크로마토그래피 정제, 동결건조 형태로 제공. 인간 시료 반응성이 높으며 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A11 |
| Immunogen | Full-length human recombinant protein of SIRT5 produced in HEK293 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
SIRT5 is a human sirtuin family member (Sir2-like proteins) found in both prokaryotic and eukaryotic organisms.
These proteins contain conserved sirtuin core domains and can metabolize NAD, potentially acting as mono-ADP-ribosyltransferases.
Sirtuins may play roles in histone deacetylation and NAD metabolism, suggesting an interaction mechanism between Sir2, NAD, and acetylated histones.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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