Thermo Fisher Scientific CD23 Monoclonal Antibody (B3B4), eFluor 660, eBioscience

Thermo Fisher Scientific CD23 Monoclonal Antibody (B3B4), eFluor 660, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• Publications: View 13 publications

Product Specifications

Available Formats

• Unconjugated
• Biotin
• Brilliant UV 805
• eFluor 450
• FITC
• NovaFluor Blue 510 / 610-70S / 660-120S
• NovaFluor Red 685 / 710 / 725
• NovaFluor Yellow 610 / 660 / 755
• PE / PE-Cyanine7 / PerCP-eFluor 710
• Super Bright 436 / 600 / 645
• Custom conjugation available

Product Specific Information

The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on resting conventional B cells and modulated upon B-cell activation. B-1 cell lineage (CD5+ B cells) does not express CD23. Soluble forms of CD23 are biologically active and act as low affinity receptors for IgE, playing a role in IgE response regulation and B-cell activation. CD21 is thought to bind CD23.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes, ≤0.5 µg/test (100 µL final volume). Cell number can range from 10⁵–10⁸ cells/test.
• Recommendation: Titrate antibody for optimal performance.
• Fluorophore Info: eFluor® 660 replaces Alexa Fluor® 647, emits at 659 nm, excited with red laser (633 nm).
• Excitation/Emission: 633–647 nm / 668 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD23 is a 45 kDa glycoprotein present on a subpopulation of freshly isolated peripheral blood and tonsil B cells, strongly expressed on EBV-transformed B lymphoblasts. It is identical to the low affinity IgE receptor found on B cells. Expression is detected in neoplastic cells from B cell chronic lymphocytic leukemia and certain lymphomas. Functionally, CD23 is involved in B cell growth, differentiation, and IgE production. Soluble CD23 acts as a potent mitogenic factor. Diseases associated include chronic conjunctivitis and chronic lymphocytic leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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