Thermo Fisher Scientific Caspase 9 Polyclonal Antibody
Rabbit polyclonal antibody targeting human Caspase 9 for apoptosis research. Validated for WB, IHC, and IP applications. High specificity and affinity-purified. Suitable for detecting Caspase 9 in cytoplasmic fractions. For research use only.
- 카탈로그번호
- 500-2294
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Caspase 9 Polyclonal Antibody
Thermo Fisher Scientific Caspase 9 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from N-terminus of human Caspase 9 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Human liver tissue
- Cellular location: Cytoplasmic
Target Information
Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 containing a CARD domain. Caspases are cysteine proteases expressed as inactive precursors that mediate apoptosis through proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, present in the cytosol, and translocates to the mitochondria upon activation. It participates in the caspase activation cascade responsible for apoptosis execution and activates Caspase 3 and Caspase 6.
Binding of Caspase 9 to Apaf-1 leads to activation of the protease, which subsequently cleaves and activates Caspase 3. Caspase 9 promotes DNA damage-induced apoptosis in an ABL1/c-Abl-dependent manner and cleaves poly(ADP-ribose) polymerase (PARP). Caspase 9 is expressed in various human tissues. In rats, an isoform has been identified with an alternative C-terminal sequence. Mutations in Caspase 9 affect brain development; in mice, insufficient Caspase 9 causes abnormal cerebrum and neuron development. In humans, dysfunctional Caspase 9 expression varies by tissue, and low levels may contribute to cancer and neurodegenerative diseases such as Alzheimer’s Disease (AD).
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
제품 이미지
(이미지 파일은 본문 하단에 위치합니다)
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.