Thermo Fisher Scientific NOB1 Monoclonal Antibody (OTI2F5), TrueMAB
NOB1 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI2F5). Western blot에 최적화되어 있으며 Human, Mouse, Rat 시료에 반응. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 포함. 연구용으로만 사용 가능.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 2–234 of human NOB1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform desalting prior to use (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing, cleaving a 20S rRNA intermediate at cleavage site D to produce mature 18S rRNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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