Thermo Fisher Scientific KCTD13 Monoclonal Antibody (OTI3B12), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Cytochrome P450 superfamily proteins are monooxygenases that catalyze multiple biochemical reactions involved in drug metabolism and synthesis of cholesterol, steroids, and lipids.
CYP4F3, a 60 kDa protein, is a novel member of this family. It contains a conserved heme-binding domain near the C-terminus and catalyzes the ω-hydroxylation of LTB4.
CYP4F3 is localized in the endoplasmic reticulum and plays a vital role in the inactivation of LTB4 and control of inflammation.

CYP4F3 exists in two functionally distinct isoforms:

• CYP4F3A: Neutrophil isoform, expressed in neutrophils and myeloid cells, catalyzes microsomal ω-hydroxylation of LTB4. Interacts with CD11b and myeloperoxidase, regulating cellular development in bone marrow.
• CYP4F3B: Liver isoform, catalyzes conversion of arachidonic acid to 20-HETE, a potent activator of PKC, implicated in cell proliferation, vascular tone, and natriuresis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.