Thermo Fisher Scientific NSE Polyclonal Antibody
Thermo Fisher Scientific의 NSE Polyclonal Antibody는 인간, 마우스, 랫트에 반응하며 Western blot 및 IHC에 적합합니다. 항원 친화 크로마토그래피로 정제된 rabbit polyclonal antibody로, NSE 단백질 검출에 유용합니다. 신경 및 신경내분비 세포 연구용으로 이상적입니다.
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- PA527452
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | - |
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse, Rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–268 of Human ENO2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.13 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C (short term). For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544928 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse brain
- Predicted reactivity: Mouse (98%), Rat (99%), Zebrafish (83%), Chicken (89%), Rhesus Monkey (100%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. NSE is highly stable in biological fluids and can diffuse into extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.
NSE, also known as enolase gamma, is expressed primarily in neurons and neuroendocrine cells. Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. Because neurons require high energy, they are rich in glycolytic enzymes such as GAPDH and NSE. Antibodies against NSE are useful for identifying neuronal cell bodies, developing neuronal lineages, and neuroendocrine cells.
Release of NSE into CSF and blood serves as a biomarker of neuronal injury. Clinically, NSE is used as a marker of neuronal damage in disorders such as stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Elevated NSE levels are also observed in certain neoplasms, including pediatric neuroblastoma and small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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