Takara His-tagged recombinant protein purification—His60 Ni Superflow Resin

His60 Ni Superflow Resin is a high-capacity Ni-IDA resin for the one-step purification of recombinant his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells. The modified Ni-IDA chemistry makes this resin one of the best on the market in terms of capacity, metal-ion leakage, and purity, when compared to other Ni-based resins such as Ni-NTA or other Ni-IDA resins. See how His60 outperforms Ni-NTA here.

His60 Ni Superflow Resin is a high-capacity Ni-IDA resin for the one-step purification of recombinant his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells. The modified Ni-IDA chemistry makes this resin one of the best on the market in terms of capacity, metal ion leakage, and purity, when compared to other Ni-based resins such as Ni-NTA or other Ni-IDA resins. See how His60 outperforms Ni-NTA here.

The recombinant his-tagged protein purification principle

His60 Ni Superflow Resin is compatible with batch/gravity flow applications, as well as with the major automated liquid chromatography systems and manual syringe processing.The resin has low metal-ion leakage and the highest binding capacity to date (outperforming Ni-NTA and other resins). The resin enables fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses. For additional information, please refer to the User Manual for Recombinant His-Tagged Protein Purification.

The recombinant his-tagged protein purification procedure

The purification of recombinant his-tagged proteins consists of 4 steps: cell lysis/extraction, binding, washing, and elution. His60 Ni Superflow Resin allows one-step protein purification under either native or denaturing conditions. The resin is compatible with multiple denaturants and detergents—refer to the User Manual for a complete reagent compatibility table. Purified recombinant his-tagged proteins can be eluted by the addition of 300 mM imidazole or by a reduction in pH.

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Overview

• Up to 60 mg/ml of recombinant his-tagged protein
• Low metal ion leakage in eluted his-tagged protein
• For purification under native or denaturing conditions
• Use for batch/gravity flow and automated FPLC purification

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Applications

Purified recombinant his-tagged proteins can be used for:

• Protein labeling
• Immunization to produce antibodies
• Animal studies
• Other applications

We also recommend

• His-Tag Detection Antibodies
  Use highly sensitive antibodies to detect his-tagged recombinant proteins in Western blot, ELISA, and immunocytohistochemical assays
• ProteoGuard Protease Inhibitor Cocktail
  Use this protease inhibitor cocktail to suppress proteolysis in all your cell lysates. We recommend using ProteoGuard with all our protein purification products

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.