Thermo Fisher Scientific Aurora B Recombinant Rabbit Monoclonal Antibody (AURKB, 3121R)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed):
  Incubate antibody for 30 minutes at room temperature.
  Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Aurora kinases associate with microtubules during chromosome movement and segregation.
AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to K-fibers.
This complex functions in chromosome alignment, separation, histone modification, and cytokinesis.

The chromosomal passenger complex (CPC) includes:

• Aurora B
• INCENP
• Survivin
• Borealin

These subunits regulate the activity, localization, and stability of Aurora B.
Two CPC forms exist during mitosis: one containing all four members, and another consisting of INCENP and Aurora B.
Aurora B is overexpressed in several tumors, including breast and colon cancers, and may be linked with Aurora-A kinase through a feedback mechanism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.