Thermo Fisher Scientific VAChT Polyclonal Antibody
Rabbit polyclonal antibody targeting VAChT for WB, IHC, ICC/IF, and ELISA applications. Reacts with human and rat proteins. Affinity purified, liquid form, unconjugated, stored at -20°C. Suitable for research use in acetylcholine transporter studies.
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Thermo Fisher Scientific VAChT Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 15 µg/mL |
| ELISA | 5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to an internal portion of rat vesicular acetylcholine transporter conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.9 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against rat VAChT. A BLAST analysis suggests cross-reactivity with mouse and human VAChT based on 100% sequence homology with the immunizing peptide.
Target Information
The VAChT gene encodes a transmembrane protein belonging to the vesicular amine transporter family. It transports acetylcholine into secretory vesicles for release into the extracellular space, utilizing a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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