Thermo Fisher Scientific CD202b (TIE2) Monoclonal Antibody (TEK4), eBioscience
Mouse Tie-2 (CD202b) 인식 단클론 항체로, 혈관형성 및 조혈 관련 연구에 적합. Flow cytometry 및 IHC(F) 등 다양한 응용 가능. 높은 순도(>90%)와 낮은 응집도(<10%)를 보유하며 4°C에서 안정적으로 보관.
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Thermo Fisher Scientific CD202b (TIE2) Monoclonal Antibody (TEK4), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 11 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 5 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 19 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse, Pig |
| Host / Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TEK4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467792 |
Additional Formats Available:
Product Specific Information
The TEK4 monoclonal antibody reacts with mouse Tie-2 (CD202), a member of the tyrosine kinase receptor family. Tie-2 is expressed on endothelial and a subset of hematopoietic cells and plays a role in angiogenesis and hematopoiesis during mouse embryonic development. In fetal liver and adult bone marrow, Tie-2 is expressed by hematopoietic progenitor cells (Lineage^-, c-Kit^+, Sca1^+). Long-term multilineage repopulating cells are found in Tie-2^+ populations but not in Tie-2^- cells.
Applications Reported: Suitable for flow cytometric analysis and immunohistology staining of frozen tissue sections.
Applications Tested: Blocking of staining with fluorochrome-conjugated TEK4 on mouse bone marrow cells. Recommended ≤1 µg/test (100 µL final volume). Cell number: 10^5–10^8 cells/test. Antibody should be titrated for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
The TEK receptor tyrosine kinase is expressed predominantly in endothelial cells. TEK signaling is essential for endothelial-smooth muscle cell communication during venous morphogenesis, and mutations are linked to inherited venous malformations. Angiopoietin-1 binds TEK to mediate vascular development signaling. TIE2 expression increases with thyroid-stimulating hormone and cAMP-elevating agents. Hematopoietic stem cells (HSCs) expressing TIE2 are quiescent, antiapoptotic, and adhere to osteoblasts in the bone marrow niche.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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