Thermo Fisher Scientific CD202b (TIE2) Monoclonal Antibody (TEK4), eBioscience

Thermo Fisher Scientific CD202b (TIE2) Monoclonal Antibody (TEK4), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available:

• APC
• Biotin
• Functional Grade
• PE
• Request custom conjugation

Product Specific Information

The TEK4 monoclonal antibody reacts with mouse Tie-2 (CD202), a member of the tyrosine kinase receptor family. Tie-2 is expressed on endothelial and a subset of hematopoietic cells and plays a role in angiogenesis and hematopoiesis during mouse embryonic development. In fetal liver and adult bone marrow, Tie-2 is expressed by hematopoietic progenitor cells (Lineage^-, c-Kit^+, Sca1^+). Long-term multilineage repopulating cells are found in Tie-2^+ populations but not in Tie-2^- cells.

Applications Reported: Suitable for flow cytometric analysis and immunohistology staining of frozen tissue sections.
Applications Tested: Blocking of staining with fluorochrome-conjugated TEK4 on mouse bone marrow cells. Recommended ≤1 µg/test (100 µL final volume). Cell number: 10^5–10^8 cells/test. Antibody should be titrated for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

The TEK receptor tyrosine kinase is expressed predominantly in endothelial cells. TEK signaling is essential for endothelial-smooth muscle cell communication during venous morphogenesis, and mutations are linked to inherited venous malformations. Angiopoietin-1 binds TEK to mediate vascular development signaling. TIE2 expression increases with thyroid-stimulating hormone and cAMP-elevating agents. Hematopoietic stem cells (HSCs) expressing TIE2 are quiescent, antiapoptotic, and adhere to osteoblasts in the bone marrow niche.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.