Thermo Fisher Scientific Interleukin-1 alpha (IL-1 alpha) Monoclonal Antibody (IL1A, 3981)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is encoded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, shared with IL-1 beta.
Located on chromosome 2q13, IL-1 alpha plays a key role in innate host defense by triggering production of other proinflammatory cytokines and initiating acute-phase responses.
Its activity is regulated by IL-1 Receptor Antagonist (IL-1RA), which blocks receptor binding via competitive inhibition.
IL-1 alpha is involved in immune responses, inflammation, and hematopoiesis. It is synthesized as a proprotein by monocytes and macrophages, processed upon cell injury, and induces apoptosis.
The IL1A gene is part of a cytokine gene cluster on chromosome 2, regulating NF-kappaB and MAPK signaling pathways. IL-1 alpha also stimulates IL6 and COX-2 (PTGS2) expression and enhances nitric oxide (NO) production.
Elevated IL-1 alpha levels are associated with chronic inflammatory diseases such as rheumatoid arthritis, psoriasis, and multiple sclerosis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.