Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), Biotin, eBioscience
인간 CD127(IL-7 수용체 α)을 인식하는 eBioRDR5 단클론 항체로, Biotin 결합형입니다. Flow cytometry에 최적화되어 있으며, 0.125 µg/test 농도로 사용 가능합니다. 면역세포 발달 및 T세포 항상성 연구에 유용합니다. Thermo Fisher Scientific 제품.
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Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), Biotin, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | View 16 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioRDR5 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657595 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 700
- APC, APC-eFluor 780
- Brilliant UV 496, 563, 737, 805
- Brilliant Violet 421, 711, 786
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Blue/Red/UV/Violet/Yellow series
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation
Product Specific Information
The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132 (common gamma chain) to form the IL-7 receptor (IL-7R).
CD127 is a type I glycoprotein (~75–80 kDa) expressed on immature B cells, thymocytes, and most mature T cells, with transient down-regulation upon activation.
IL-7 binding triggers signaling via tyrosine kinase pathways, including Jak/STAT. IL-7 is crucial for lymphocyte development and T-cell homeostasis.
CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (Tregs). CD4+CD25+CD127^low cells are highly enriched for regulatory T cells expressing Foxp3 and showing suppressive activity in vitro.
Binding of eBioRDR5 to PBMCs is blocked by pre-incubation with recombinant human IL-7 (Product #14-1079-80).
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on normal human peripheral blood cells at ≤0.125 µg/test (in 100 µL final volume).
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD127 (IL-7 receptor α) is a glycoprotein regulating lymphopoiesis. The active receptor is an IL7R α/γ heterodimer.
The γ-chain activates signal transduction, while the α-chain mediates specific signaling via cytoplasmic molecules.
CD127 promotes proliferation of precursor B cells, thymocytes, T-cell progenitors, and mature CD4+/CD8+ T cells.
Dysfunction of CD127 is associated with severe combined immunodeficiency (SCID).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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