Thermo Fisher Scientific Phospho-MAP2K1/MAP2K2 (Ser222) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human MAP2K1(Accession Q02750), corresponding to amino acid residues around phosphorylated Ser222. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MEK1/2,uniProtId:Q02750-1,ncbiNodeId:9606,antigenRange:222,antigenLength:393,antigenImageFileName:PA5-104956_MEK12_Q02750-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104956_MEK12_Q02750-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:MEK1/2,uniProtId:P36507-1,ncbiNodeId:9606,antigenRange:222,antigenLength:400,antigenImageFileName:PA5-104956_MEK12_P36507-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104956_MEK12_P36507-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816429

Product Specific Information

Antibody detects endogenous levels of MEK1/2 only when phosphorylated at Ser222, which site historically referenced as Ser221.

Target Information

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). In brain, this pathway also plays a key role in synaptic (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.