Thermo Fisher Scientific CNTF Polyclonal Antibody, PeproTech
Rabbit에서 생산된 Human CNTF 특이 Polyclonal Antibody로, Western blot, ELISA, Immunostaining 등에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS 내 동결건조 형태로 제공. 신경세포 생존 및 성장 관련 연구에 적합.
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CNTF Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| ELISA | 0.5–2.0 µg/mL | - |
| Blocking Assay (BLOCK) | - | 1 publication |
| In vitro Assay (IV) | - | 1 publication |
| Immunostaining (IS) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse, Rat |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human CNTF |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929447 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
AFTEHSPLTP HRRDLCSRSI WLARKIRSDL TALTQSYVKH QGLNKNINLD SADGMPVAST DQWSQLTQAQ RLQQNLQAYR TFHVLLARLL QDQQVHFTPT QGDFHQAIHT LLLQVAAFAY QIQQLMILLQ YKIPRNQADG MPINVGDGGL FQKKLWGLKV LQQLSQWTVR SIHDLRFISS HQTGIPARGS HYIANNKKM.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human CNTF. Anti-Human CNTF-specific antibody was purified by affinity chromatography employing an immobilized Human CNTF matrix.
Sandwich ELISA:
To detect Human CNTF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human CNTF (500-P140Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human CNTF.
Western Blot:
To detect Human CNTF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human CNTF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The protein encoded by this gene is a polypeptide hormone whose actions are restricted to the nervous system, where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It acts as a potent survival factor for neurons and oligodendrocytes and may help reduce tissue destruction during inflammatory attacks. A mutation in this gene causing aberrant splicing leads to ciliary neurotrophic factor deficiency, though not directly linked to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequences has been identified but is thought to be non-coding. Similar read-through transcription has been observed in mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.