Thermo Fisher Scientific Zap-70 Monoclonal Antibody (J.947.4)
ZAP-70 단백질을 인식하는 Rabbit monoclonal antibody (Clone J.947.4). Western blot, IHC, ICC, Flow cytometry, IP에 적합. Human, Mouse, Rat 반응성. 액상 형태, -20°C 보관. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:800 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:200 |
| Immunoprecipitation (IP) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | J.947.4 |
| Immunogen | Synthetic peptide corresponding to residues surrounding the amino-terminus of human Zap-70 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 142 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES (pH 7.5), 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10982111 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family, expressed exclusively in T-cells and natural killer cells, and essential for T-cell receptor activation. Upon TCR engagement, ZAP-70 is phosphorylated on tyrosines 292, 315, and 319. Phosphorylation at tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances signaling, while phosphorylation at tyrosine 292 attenuates activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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